재조합 Alcaligenes eutrophus 균주 개발 및 Poly(3-hydroxybutyrate) 생산에 관한 연구 Development of Recombinant Alcaligenes eutrophus for the Production of Poly(3-hydroxybutyrate)

In order to improve the productivity of poly(3-hydroxybutyrate)[P(3HB)], Alcaligenes eutrophus was metabolically engineered tn amplify the activities of the three enzymes involved in the synthesis of P(3HB). The A. eutrophus P(3HB) biosynthesis genes coding for P(3HB) synthase, β-ketothiolase, and reductase were cloned into a broad-host-range plasmid pVK101. Recombinant A. eutrophus strain was developed by transforming with this plasmid by electroporation. The efficiency of transformation was in an order of 10³ transformants/㎍ DNA. In flask cultures, the final cell concentration of recombinant A. eutrophus decreased with increasing carbon/nitrogen(C/N) molar ratio, On the other hand, P(3HB) concentration was highest at the medium C/N molar ratio. For the fixed nitrogen concentration, the concentrations of cell and P(3HB) increased with increasing glucose concentration. Comparison of cell growth and P(3HB) production by recombinant and wild type A. eutrophu. in batch culture showed that the final P(3HB) concentration, P(3HB) content, and P (3HB) synthesis rate were all higher in the recombinant strain compared with the wild type.
Publisher
한국화학공학회
Issue Date
1997-10
Language
KOR
Citation

화학공학회지, v.35, no.5, pp.684 - 689

ISSN
0256-1107
URI
http://hdl.handle.net/10203/75049
Appears in Collection
CBE-Journal Papers(저널논문)
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