PRODUCTION OF POLY(3-HYDROXYBUTYRIC ACID) BY RECOMBINANT ESCHERICHIA-COLI STRAINS - GENETIC AND FERMENTATION STUDIES

A number of Escherichia coli strains including K12, B, W, XL1-Blue, DH5 alpha, HB101, JM109, and C600 were transformed with the stable high-copy-number plasmid pSYL105 containing the Alcaligenes eutrophus polyhydroxyalkanoic acid biosynthesis genes, and were subsequently compared for their ability to synthesize and accumulate poly(3-hydroxybutyric acid) (PHB). The rate of PHB synthesis, the extent of PHB accumulation, and PHB yield from glucose varied considerably from one strain to another. Strains XL1-Blue and B harboring pSYL105 synthesized PHB at the highest rate to a final concentration of ca. 7 g/L in complex medium containing 20 g glucose/L. With an aim to reduce the cost of the medium, the effect on PHB accumulation of supplementing a defined medium with complex nitrogen sources was examined. A PHB concentration of 81 g/L could be obtained in 41 h from a pH-stat fed-batch culture of XL1-Blue(pSYL105) in a semidefined medium. When the availability of acetyl-CoA was increased by supplementing the medium with complex nitrogen sources, amino acids, or oleic acid, PHB synthesis by recombinant E. coli was enhanced.
Publisher
CANADIAN SCIENCE PUBLISHING
Issue Date
1995
Language
ENG
Keywords

POLY-BETA-HYDROXYBUTYRATE; ALCALIGENES-EUTROPHUS H16; CELL-DENSITY CULTIVATION; FED-BATCH CULTURE; PHB; IDENTIFICATION; STABILIZATION; STABILITY; PATHWAY; SUCROSE

Citation

CANADIAN JOURNAL OF MICROBIOLOGY, v.41, pp.207 - 215

ISSN
0008-4166
URI
http://hdl.handle.net/10203/75035
Appears in Collection
CBE-Journal Papers(저널논문)
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