Hepatitis C virus NS5A protein is phosphorylated by casein kinase II

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dc.contributor.authorKim, Jiyunko
dc.contributor.authorLee, Daeyoupko
dc.contributor.authorChoe, Joonhoko
dc.date.accessioned2013-03-02T18:10:54Z-
dc.date.available2013-03-02T18:10:54Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued1999-04-
dc.identifier.citationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.257, no.3, pp.777 - 781-
dc.identifier.issn0006-291X-
dc.identifier.urihttp://hdl.handle.net/10203/74846-
dc.description.abstractHepatitis C virus (HCV) has a positive-strand RNA genome that encodes a polyprotein, which is posttranslationally processed by cellular and viral proteinases into three structural and six non-structural (NS) proteins. The NS5A protein, expressed in mammalian cells, exists as two phosphorylated forms of 56 kDa and 58 kDa. In this study, we provide evidence for a stable association between NS5A and a protein kinase from rat-1 cells by affinity to immobilized glutathione-S-transferase (GST)-NS5A fusion protein. This protein kinase was associated through the N-terminus of NS5A and was not regulated by cell cycle. The GST-NS5A was also phosphorylated in vitro by the purified casein kinase II (CKII), a member of the CMCG kinase family. Since CKII and the NS5A-associated protein kinase have the same molecular size and property by In-gel kinase assay and an inhibitor treatment test, we conclude that HCV NS5A protein is phosphorylated by CKII. (C) 1999 Academic Press.-
dc.languageEnglish-
dc.publisherACADEMIC PRESS INC-
dc.subjectPOLYPROTEIN-
dc.subjectREGION-
dc.subjectGENOME-
dc.subjectINVITRO-
dc.subjectSUBUNIT-
dc.subjectNS3-
dc.titleHepatitis C virus NS5A protein is phosphorylated by casein kinase II-
dc.typeArticle-
dc.identifier.wosid000079925300023-
dc.identifier.scopusid2-s2.0-0033590926-
dc.type.rimsART-
dc.citation.volume257-
dc.citation.issue3-
dc.citation.beginningpage777-
dc.citation.endingpage781-
dc.citation.publicationnameBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.contributor.localauthorLee, Daeyoup-
dc.contributor.localauthorChoe, Joonho-
dc.contributor.nonIdAuthorKim, Jiyun-
dc.type.journalArticleArticle-
dc.subject.keywordPlusPOLYPROTEIN-
dc.subject.keywordPlusREGION-
dc.subject.keywordPlusGENOME-
dc.subject.keywordPlusINVITRO-
dc.subject.keywordPlusSUBUNIT-
dc.subject.keywordPlusNS3-
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