DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jiyun | ko |
dc.contributor.author | Lee, Daeyoup | ko |
dc.contributor.author | Choe, Joonho | ko |
dc.date.accessioned | 2013-03-02T18:10:54Z | - |
dc.date.available | 2013-03-02T18:10:54Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1999-04 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.257, no.3, pp.777 - 781 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/10203/74846 | - |
dc.description.abstract | Hepatitis C virus (HCV) has a positive-strand RNA genome that encodes a polyprotein, which is posttranslationally processed by cellular and viral proteinases into three structural and six non-structural (NS) proteins. The NS5A protein, expressed in mammalian cells, exists as two phosphorylated forms of 56 kDa and 58 kDa. In this study, we provide evidence for a stable association between NS5A and a protein kinase from rat-1 cells by affinity to immobilized glutathione-S-transferase (GST)-NS5A fusion protein. This protein kinase was associated through the N-terminus of NS5A and was not regulated by cell cycle. The GST-NS5A was also phosphorylated in vitro by the purified casein kinase II (CKII), a member of the CMCG kinase family. Since CKII and the NS5A-associated protein kinase have the same molecular size and property by In-gel kinase assay and an inhibitor treatment test, we conclude that HCV NS5A protein is phosphorylated by CKII. (C) 1999 Academic Press. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC | - |
dc.subject | POLYPROTEIN | - |
dc.subject | REGION | - |
dc.subject | GENOME | - |
dc.subject | INVITRO | - |
dc.subject | SUBUNIT | - |
dc.subject | NS3 | - |
dc.title | Hepatitis C virus NS5A protein is phosphorylated by casein kinase II | - |
dc.type | Article | - |
dc.identifier.wosid | 000079925300023 | - |
dc.identifier.scopusid | 2-s2.0-0033590926 | - |
dc.type.rims | ART | - |
dc.citation.volume | 257 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 777 | - |
dc.citation.endingpage | 781 | - |
dc.citation.publicationname | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.contributor.localauthor | Lee, Daeyoup | - |
dc.contributor.localauthor | Choe, Joonho | - |
dc.contributor.nonIdAuthor | Kim, Jiyun | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | POLYPROTEIN | - |
dc.subject.keywordPlus | REGION | - |
dc.subject.keywordPlus | GENOME | - |
dc.subject.keywordPlus | INVITRO | - |
dc.subject.keywordPlus | SUBUNIT | - |
dc.subject.keywordPlus | NS3 | - |
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