cAMP response element-binding protein-binding protein binds to human papillomavirus E2 protein and activates E2-dependent transcription

cAMP response element-binding protein-binding protein (CBP) is a eucaryotic transcriptional co-activator that contains multiple protein-protein interaction domains for association with various transcription factors, components of the basal transcriptional apparatus, and other co-activator proteins, Here, we report that CBP is also a co-activator of the human papillomavirus (HPV) E2 protein, which is a sequence-specific transcription/replication factor. We provide biochemical, genetic, and functional evidence that CBP binds directly to HPV E2 in vivo and in vitro and activates Ea-dependent transcription. Mutations in an amphipathic helix within HPV-18 E2 abolish its transcriptional activation properties and its ability to bind to CBP, Furthermore, the binding of CBP to E2 was shown to be necessary for Ea-dependent transcription. Interestingly, the histone acetyltransferase activity of CBP plays a role in CBP activation of Ea-dependent transcription.
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Issue Date
2000-03
Language
ENG
Keywords

LARGE-T-ANTIGEN; ADENOVIRUS E1A; HISTONE ACETYLTRANSFERASE; REPLICATION FUNCTIONS; REMODELING FACTOR; IN-VITRO; TRANSACTIVATION DOMAIN; DNA-REPLICATION; TERMINAL DOMAIN; CBP

Citation

JOURNAL OF BIOLOGICAL CHEMISTRY, v.275, no.10, pp.7045 - 7051

ISSN
0021-9258
URI
http://hdl.handle.net/10203/74845
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
  • Hit : 176
  • Download : 0
  • Cited 0 times in thomson ci
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡClick to seewebofscience_button
⊙ Cited 71 items in WoSClick to see citing articles inrecords_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0