DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, YS | ko |
dc.contributor.author | Kim, SY | ko |
dc.contributor.author | Kim, JH | ko |
dc.contributor.author | Kim, Sun-Chang | ko |
dc.date.accessioned | 2013-03-02T17:54:50Z | - |
dc.date.available | 2013-03-02T17:54:50Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1999-01 | - |
dc.identifier.citation | JOURNAL OF BIOTECHNOLOGY, v.67, no.2-3, pp.159 - 171 | - |
dc.identifier.issn | 0168-1656 | - |
dc.identifier.uri | http://hdl.handle.net/10203/74805 | - |
dc.description.abstract | Xylitol production from xylose was studied using recombinant Saccharomyces cerevisiae 2805 containing xylose reductase genes (XYLI) of Pichia stipitis at chromosomal delta-sequences. S. cerevisiae 2805-39-40, which contains about 40 copies of the XYL1 gene on the chromosome, was obtained by a sequential transformation using a dominant selection marker neo(r) and an auxotrophic marker URA3. The multiple XYLI genes were stably maintained on the chromosome even after 21 and IO days in the non-selective sequential batch and chemostat cultures, respectively, whereas S. cerevisiae 2805:pVTXR, which harbors the episomal plasmid pVTXR having the XYLI gene, showed mitotic plasmid instability and more than 95% of the cells lost the plasmid under the same culture conditions. In the first batch (3 days) of the sequential batch culture, volumetric xylitol productivity was 0.18 g l(-1) h(-1) for S. cerevisiae 2805-39-40, as compared to 0.21 g l(-1) h(-1) for S. cerevisiae 2805:pVTXR. However, the xylitol productivity of the latter started to decrease rapidly in the third batch and dropped to 0.04 g l(-1) h(-1) in the seventh batch, whereas the former maintained the stable xylitol productivity at 0.18 g l(-1) h(-1) through the entire sequential batch culture. The xylitol production level in the chemostat culture was about 8 g l(-1) for S. cerevisiae 2805-39-40, as compared to 2.0 g l(-1) for S. cerevisiae 2805:pVTXR after 10 days of cultures even though the xylitol production level of the latter was higher than that of the former for the first 5 days. The results of this experiment indicate that S. cerevisiae containing the multiple XYLI genes on the chromosome is much more efficient for the xylitol production in the long-term non-selective culture than S. cerevisiae harboring the episomal plasmid containing the XYLI gene. (C) 1999 Elsevier Science B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | XYL1 GENE | - |
dc.subject | HIGH COPY | - |
dc.subject | YEAST | - |
dc.subject | STABILITY | - |
dc.subject | INTEGRATION | - |
dc.subject | EXPRESSION | - |
dc.subject | SECRETION | - |
dc.subject | TRANSFORMATION | - |
dc.subject | COSUBSTRATE | - |
dc.subject | VECTORS | - |
dc.title | Xylitol production using recombinant Saccharomyces cerevisiae containing multiple xylose reductase genes at chromosomal delta-sequences | - |
dc.type | Article | - |
dc.identifier.wosid | 000078602000006 | - |
dc.identifier.scopusid | 2-s2.0-0032945807 | - |
dc.type.rims | ART | - |
dc.citation.volume | 67 | - |
dc.citation.issue | 2-3 | - |
dc.citation.beginningpage | 159 | - |
dc.citation.endingpage | 171 | - |
dc.citation.publicationname | JOURNAL OF BIOTECHNOLOGY | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.nonIdAuthor | Kim, YS | - |
dc.contributor.nonIdAuthor | Kim, SY | - |
dc.contributor.nonIdAuthor | Kim, JH | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | recombinant yeast | - |
dc.subject.keywordAuthor | xylose reductase gene | - |
dc.subject.keywordAuthor | xylitol | - |
dc.subject.keywordAuthor | delta-sequence | - |
dc.subject.keywordAuthor | plasmid stability | - |
dc.subject.keywordPlus | XYL1 GENE | - |
dc.subject.keywordPlus | HIGH COPY | - |
dc.subject.keywordPlus | YEAST | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | INTEGRATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordPlus | TRANSFORMATION | - |
dc.subject.keywordPlus | COSUBSTRATE | - |
dc.subject.keywordPlus | VECTORS | - |
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