Decreased chimeric antibody productivity of KR12H-1 transfectoma during long-term culture results from decreased antibody gene copy number

The stability of KR12H-1 transfectoma in regard to chimeric antibody production was examined during long-term, repeated batch culture without selection pressure using antibiotics. Both serum-supplemented and serum-free media were used. Regardless of the medium used, the specific antibody productivity (q(Ab)) Of transfectoma decreased by 60% to 88% during 70-day culture. This loss of antibody productivity was not due mainly to the appearance of a nonproducing population (NP) of transfectoma. The percentage of a producing population (P), which was monitored by the limiting dilution method, remained over 90% until the end of culture, indicating that the q(Ab) of P decreased during the culture. Flow cytometric data also showed the increase of cell population with low fluorescence intensity during culture, indicating that the intracellular antibody content of P decreased. The subclones of P obtained at the end of long-term culture were further characterized. Compared with the q(Ab) of P at the beginning of long-term culture, the q(Ab) of most P subclones was significantly low, confirming that the loss of antibody productivity was due mainly to the decreased q(Ab) of P during long-term culture. The decreased antibody gene copy number of P subclones was found to be partly responsible for the decreased q(Ab) of P during long-term culture. (C) 1996 John Wiley & Sons, Inc.
Publisher
JOHN WILEY SONS INC
Issue Date
1996-08
Language
English
Keywords

HYBRIDOMA CELL-LINE; HEPATITIS-B VIRUS; SERUM-FREE MEDIA; STABILITY; ANTIGEN; MOUSE; SPECIFICITY; SECRETION; MYELOMAS; CLONING

Citation

BIOTECHNOLOGY AND BIOENGINEERING, v.51, no.4, pp.479 - 487

ISSN
0006-3592
URI
http://hdl.handle.net/10203/74795
Appears in Collection
BS-Journal Papers(저널논문)
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