Expression of a non-inactivating K+ channel driven by a rat heat shock promoter increased the resting potential in Aplysia silent neurons

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We assessed the role of a non-inactivating K+ channel (aKv5.1) in the resting potential by overexpressing this channel by heat shock in the neurons. A reporter gene lacZ linked to a promoter region spanning from the - 285 to the + 88 base of the rat HSP70ib gene was induced 62.5-fold when this DNA construct was microinjected into the neurons of the marine mollusk Aplysia and treated with heat shock at 30 degrees C for 3 h. Using this efficient induction system, we induced the expression of aKv5.1 by heat shock in cultured, electrically silent neurons of Aplysia and examined its effect on the resting potential. The channel expression increased the resting potential by approximately 10 mV. This increase was specific to heat shock induction and abolished by treatment with TEA, a specific K+ channel blocker. These results provide the direct evidence that a low voltage-activated, non-inactivating K+ channel can contribute to the resting potential. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
Publisher
ELSEVIER SCI IRELAND LTD
Issue Date
1999-05
Language
ENG
Article Type
Article
Keywords

DISSOCIATED CELL-CULTURE; SENSORY NEURONS; NERVOUS-SYSTEM; HSP70 GENE; SHAKER; MODULATION; RELEASE; CLONING; PROTEIN

Citation

NEUROSCIENCE RESEARCH, v.34, no.1, pp.13 - 19

ISSN
0168-0102
DOI
10.1016/S0168-0102(99)00027-9
URI
http://hdl.handle.net/10203/74776
Appears in Collection
BS-Journal Papers(저널논문)
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