DC Field | Value | Language |
---|---|---|
dc.contributor.author | Van Wagoner, NJ | ko |
dc.contributor.author | Choi, Chulhee | ko |
dc.contributor.author | Repovic, P | ko |
dc.contributor.author | Benveniste, EN | ko |
dc.date.accessioned | 2013-03-02T17:45:39Z | - |
dc.date.available | 2013-03-02T17:45:39Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2000-08 | - |
dc.identifier.citation | JOURNAL OF NEUROCHEMISTRY, v.75, no.2, pp.563 - 575 | - |
dc.identifier.issn | 0022-3042 | - |
dc.identifier.uri | http://hdl.handle.net/10203/74775 | - |
dc.description.abstract | Oncostatin M (OSM) is a member of the interleukin (IL)-6 family of cytokines and has both pro- and antiinflammatory properties. Of interest, OSM has functional effects within the CNS. We have shown recently that OSM can modulate expression of the cytokine IL-6 in astrocytes, Herein we characterize the molecular mechanisms and signaling cascades involved in this response. OSM induces IL-6 protein expression in a dose- and time-dependent manner in astrocytes, In addition, OSM can synergize with the cytokines tumor necrosis factor-alpha, IL-1 beta, and transforming growth factor-beta for enhanced IL-6 expression. Using neutralizing antibodies to gp130, the OSM receptor (OSMR), and the leukemia inhibitory factor receptor (LIFR), we document that OSM exclusively uses the OSMR/gp130 heterodimer in signaling events, rather than the LIFR/gp130 heterodimer. Kinetic analysis of OSM-induced IL-6 mRNA reveals two up-regulatory events. The first, peaking at 1 h, is transient, does not require protein synthesis, and is regulated at the transcriptional level. The second, peaking between 6 and 8 h, is prolonged and sensitive to puromycin, suggesting a requirement for de novo protein synthesis, and also is transcriptionally regulated. OSM-induced IL-6 mRNA and protein expression is inhibited by the mitogen-activated protein kinase (MAPK) inhibitors U0126 and SB202190, suggesting a requirement for the MAPKs ERK1/2 and p38 in this response. Finally, we show that the MAPKs ERK1/2 and p38 are activated by OSM in astrocytes and that this activation is reduced by the MAPK inhibitors. These data demonstrate that OSM induces IL-6 expression in astrocytes and that the MAPKs ERK1/2 and p38 participate in this response. | - |
dc.language | English | - |
dc.publisher | LIPPINCOTT WILLIAMS WILKINS | - |
dc.subject | LEUKEMIA INHIBITORY FACTOR | - |
dc.subject | TUMOR-NECROSIS-FACTOR | - |
dc.subject | MHC GENE-EXPRESSION | - |
dc.subject | VASOACTIVE-INTESTINAL-PEPTIDE | - |
dc.subject | MESSENGER-RNA EXPRESSION | - |
dc.subject | CLASS-II TRANSACTIVATOR | - |
dc.subject | SOLUBLE IL-6 RECEPTOR | - |
dc.subject | GROWTH-FACTOR-BETA | - |
dc.subject | TGF-BETA | - |
dc.subject | TNF-ALPHA | - |
dc.title | Oncostatin M regulation of interleukin-6 expression in astrocytes: Biphasic regulation involving the mitogen-activated protein kinases ERK1/2 and p38 | - |
dc.type | Article | - |
dc.identifier.wosid | 000088231600014 | - |
dc.identifier.scopusid | 2-s2.0-0033929853 | - |
dc.type.rims | ART | - |
dc.citation.volume | 75 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 563 | - |
dc.citation.endingpage | 575 | - |
dc.citation.publicationname | JOURNAL OF NEUROCHEMISTRY | - |
dc.identifier.doi | 10.1046/j.1471-4159.2000.0750563.x | - |
dc.contributor.localauthor | Choi, Chulhee | - |
dc.contributor.nonIdAuthor | Van Wagoner, NJ | - |
dc.contributor.nonIdAuthor | Repovic, P | - |
dc.contributor.nonIdAuthor | Benveniste, EN | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | brain | - |
dc.subject.keywordAuthor | neuroimmunology | - |
dc.subject.keywordAuthor | cytokines | - |
dc.subject.keywordAuthor | second messengers | - |
dc.subject.keywordAuthor | astrocytes | - |
dc.subject.keywordPlus | LEUKEMIA INHIBITORY FACTOR | - |
dc.subject.keywordPlus | TUMOR-NECROSIS-FACTOR | - |
dc.subject.keywordPlus | MHC GENE-EXPRESSION | - |
dc.subject.keywordPlus | VASOACTIVE-INTESTINAL-PEPTIDE | - |
dc.subject.keywordPlus | MESSENGER-RNA EXPRESSION | - |
dc.subject.keywordPlus | CLASS-II TRANSACTIVATOR | - |
dc.subject.keywordPlus | SOLUBLE IL-6 RECEPTOR | - |
dc.subject.keywordPlus | GROWTH-FACTOR-BETA | - |
dc.subject.keywordPlus | TGF-BETA | - |
dc.subject.keywordPlus | TNF-ALPHA | - |
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