Fas ligand and Fas are expressed constitutively in human astrocytes and the expression increases with IL-1, IL-6, TNF-alpha, or IFN-gamma

Fas ligand (FasL) and Fas are mediators of apoptosis, which are implicated in the peripheral deletion of autoimmune cells, activation-induced T cell death, and cytotoxicity mediated by CD8(+) T cells. Fas is also believed to be involved in several central nervous system diseases, but until now, the effector cells expressing Fast in the brain have not been identified. We investigated the expression levels of Fas and Fast with the stimulation of cytokines and the possible effector cells targeting Fas-bearing cells. Our data demonstrated that: 1) Fast is expressed constitutively on astrocytes taken from a fetus or an adult and that its expression increases when these cells are treated with IL-1, IL-6, or TNF-alpha in which the pretreatment of IFN-gamma triggers astrocytes to express more Fast; 2) astrocytes induce apoptosis in MOLT4 cells through Fast; 3) Fas is also expressed constitutively and is upregulated by IL-1, IL-6, or TNP-alpha in which the pretreatment of IFN-gamma triggers astrocytes to express more Fas; 4) apoptosis occurs when fetal astrocytes are treated with agonistic: anti-Fas IgM Ab after culture with IFN-gamma and TNF-alpha; and 5) TNF-related apoptosis inducing ligand is up-regulated in fetal astrocytes with stimuli of IL-1 or TNF-alpha. These findings suggest a possible role of astrocytes in the induction of apoptosis in central nervous system diseases.
Publisher
AMER ASSOC IMMUNOLOGISTS
Issue Date
1999-02
Language
ENG
Keywords

MULTIPLE-SCLEROSIS BRAIN; CELL-DEATH; IMMUNE PRIVILEGE; INDUCED APOPTOSIS; CD95; RECEPTOR; INVOLVEMENT; ACTIVATION; ANTIGEN; SYSTEM

Citation

JOURNAL OF IMMUNOLOGY, v.162, no.4, pp.1889 - 1895

ISSN
0022-1767
URI
http://hdl.handle.net/10203/74759
Appears in Collection
BiS-Journal Papers(저널논문)CH-Journal Papers(저널논문)
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