PRODUCTION OF SCOPOLAMINE BY NORMAL ROOT CULTURE OF HYOSCYAMUS-NIGER

Normal root of Hyoscyamus niger was cultivated in various nutrient conditions to optimize root growth and scopolamine production. Nitrate or phosphate concentration had little effect On root growth, while the low levels of nitrate or phosphate enhanced specific scopolamine content by 44% and 39%, respectively. Glucose or fructose was not adequate for the root culture as a carbon source, while the root growth was markedly enhanced at high sucrose concentration (up to 9%). The growth rate (0.59g dw./L.d.) and scopolamine productivity (1.98 mg/L.d.) was increased greater in roots with 9% initial sucrose concentration as compared to 3% sucrose (0.28 g dw./L.d., 0.98 mg/L.d.)
Publisher
CHAPMAN HALL LTD
Issue Date
1995-09
Language
ENG
Keywords

TROPANE ALKALOID PRODUCTION; SUSPENSION-CULTURES; CATHARANTHUS-ROSEUS; DATURA-STRAMONIUM; GROWTH

Citation

BIOTECHNOLOGY LETTERS, v.17, no.9, pp.921 - 926

ISSN
0141-5492
URI
http://hdl.handle.net/10203/74754
Appears in Collection
CBE-Journal Papers(저널논문)
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