Production of L-DOPA(3,4-dihydroxyphenyl-L-alanine) from benzene by using a hybrid pathway

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As an alternative approach to the production of L-DOPA from a cheap raw material, we constructed a hybrid pathway consisting of toluene dioxygenase, toluene cis-glycol dehydrogenase, and tyrosine phenol-lyase. In this pathway, catechol is formed from benzene through the sequential action of toluene dioxygenase and toluene cis-glycol dehydrogenase, and L-DOPA is synthesized from the resulting catechol in the presence of pyruvate and ammonia by tyrosine phenol-lyase cloned from Citrobacter freundii. When the hybrid pathway was expressed in E. coil, production of L-DOPA was as low as 3 mM in 4 h due to the toxic effect of benzene on the cells. In order to reduce lysis of cells, Pseudomonas aeruginosa was employed as an alternative, which resulted in accumulation of about 14 mM L-DOPA in 9 h, showing a stronger resistance to benzene. (C) 1998 John Wiley & Sons, Inc.
Publisher
JOHN WILEY SONS INC
Issue Date
1998-04
Language
English
Article Type
Article
Keywords

NUCLEOTIDE-SEQUENCE; PSEUDOMONAS-PUTIDA; ESCHERICHIA-COLI; TOLUENE; CATECHOLS

Citation

BIOTECHNOLOGY AND BIOENGINEERING, v.58, no.2-3, pp.339 - 343

ISSN
0006-3592
URI
http://hdl.handle.net/10203/73876
Appears in Collection
CH-Journal Papers(저널논문)BS-Journal Papers(저널논문)
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