DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee D.C. | ko |
dc.contributor.author | Kim G.J. | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.contributor.author | Cha Y.K. | ko |
dc.contributor.author | Lee C.Y. | ko |
dc.date.accessioned | 2013-03-02T13:14:27Z | - |
dc.date.available | 2013-03-02T13:14:27Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1997 | - |
dc.identifier.citation | BIOTECHNOLOGY AND BIOENGINEERING, v.56, no.4, pp.449 - 455 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | http://hdl.handle.net/10203/73675 | - |
dc.description.abstract | D-Hydantoinase is an industrial enzyme widely used for the synthesis of optically active D-amino acids. A gene encoding thermostable D-hydantoinase of Bacillus stearothermophilus SD-1 has previously been cloned and constitutively expressed by its native promoter in Escherichia coli XL1-Blue (Lee et al., 1996b). In this work, we attempted mass production of the D-hydantoinase by batch culture of the recombinant E. coli using glycerol as a carbon source. The plasmid content in cells increased in proportion to the culture temperature, which resulted in a two- or three-fold increase of the specific D-hydantoinase activity at 37 degrees C compared with that at 30 degrees C. The plasmid was stably maintained over 80 generations. When glycerol was initially added to a concentration of 100 g/L, the final biomass concentration reached about 50 g-dry cell weight/L in a 50 L-scale fermentation, resulting in the specific enzyme production of 3.8 x 10(4) unit/g-dry cell weight in a soluble form. Glycerol-using batch cultivation of recombinant E. coli was found to be a cost-effective process for the mass production of industrially useful D-hydantoinase. (C) 1997 John Wiley & Sons, Inc. | - |
dc.language | English | - |
dc.publisher | JOHN WILEY & SONS INC | - |
dc.subject | CELL-DENSITY CULTIVATION | - |
dc.subject | BACILLUS-STEAROTHERMOPHILUS SD-1 | - |
dc.subject | CATABOLITE REPRESSION | - |
dc.subject | ACETATE | - |
dc.subject | GROWTH | - |
dc.subject | MICROORGANISMS | - |
dc.subject | EXCRETION | - |
dc.subject | SUBJECT | - |
dc.subject | GLUCOSE | - |
dc.subject | ENZYME | - |
dc.title | Mass production of thermostable D-hydantoinase by batch culture of recombinant Escherichia coli with a constitutive expression system | - |
dc.type | Article | - |
dc.identifier.wosid | A1997YC15800010 | - |
dc.identifier.scopusid | 2-s2.0-0342506505 | - |
dc.type.rims | ART | - |
dc.citation.volume | 56 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 449 | - |
dc.citation.endingpage | 455 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.identifier.doi | 10.1002/(SICI)1097-0290(19971120)56:4<449::AID-BIT10>3.0.CO;2-7 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.nonIdAuthor | Lee D.C. | - |
dc.contributor.nonIdAuthor | Kim G.J. | - |
dc.contributor.nonIdAuthor | Cha Y.K. | - |
dc.contributor.nonIdAuthor | Lee C.Y. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | thermostable D-hydantoinase | - |
dc.subject.keywordAuthor | recombinant E-coli | - |
dc.subject.keywordAuthor | constitutive expression | - |
dc.subject.keywordAuthor | glycerol | - |
dc.subject.keywordAuthor | batch cultivation | - |
dc.subject.keywordPlus | CELL-DENSITY CULTIVATION | - |
dc.subject.keywordPlus | BACILLUS-STEAROTHERMOPHILUS SD-1 | - |
dc.subject.keywordPlus | CATABOLITE REPRESSION | - |
dc.subject.keywordPlus | ACETATE | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | MICROORGANISMS | - |
dc.subject.keywordPlus | EXCRETION | - |
dc.subject.keywordPlus | SUBJECT | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordPlus | ENZYME | - |
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