Regulation of the neuron-specific exon of clathrin light chain B

Clathrin light chain B (LCB) is a major component of clathrin coated vesicles, which are structures involved in intracellular transport. A neuron-specific isoform of LCB is generated by incorporation of a single exon (EN) using an alternative splicing mechanism that reflects the special demands of neurons, such as axonal transport and synaptic neurotransmission. Here, we demonstrate that this 1 neuron-specific exon is developmentally regulated and is excluded in non-neuronal cells because its 5' and 3' splice sites deviate from the mammalian consensus sequences. A gel retardation assay indicated the presence of a developmentally regulated factor in brain that binds to the neuronal exon. In addition, EN usage is repressed by increasing the concentration of htra2-betal, a splice factor whose isoform expression is influenced by neuronal activity. We propose that a brain-specific factor is involved in EN recognition during development and adulthood. In addition, ubiquitously expressed splicing factors such as htra2-betal are involved in regulating EN expression in the adult brain. (C) 1999 Elsevier Science B.V. All rights reserved.
Publisher
ELSEVIER SCIENCE BV
Issue Date
1999-01
Language
ENG
Keywords

PRE-MESSENGER-RNA; TRACT BINDING-PROTEIN; SPLICING FACTORS; GENE-EXPRESSION; NMDA RECEPTOR; IN-VIVO; CELL; BRAIN; DROSOPHILA; INTRON

Citation

MOLECULAR BRAIN RESEARCH, v.64, no.1, pp.108 - 118

ISSN
0169-328X
DOI
10.1016/S0169-328X(98)00313-1
URI
http://hdl.handle.net/10203/73629
Appears in Collection
BS-Journal Papers(저널논문)
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