Differential changes of CDK activities in glomeruli and tubules during the active DNA synthetic period after ischemic injury

The present study was designed to determine the spatial correlation among extent of DNA synthetic activity, expressions of G(1)/S phase cyclins, cyclin-dependent kinases (CDKs) and CIP/KIP family of CDK inhibitors (CKIs), and activities of G(1)/S phase CDKs in glomeruli and outer medullae of kidneys during the active regeneration period after ischemic injury. DNA synthetic activity was measured using [H-3]-thymidine autoradiogram in the kidney sections. Cyclin, CDK, and CKI proteins were determined by Western blot analysis. CDK activities were determined by phosphorylation amount using specific substrate. The protein levels of cyclins (D1, D3, E, A) and activities of CDK4 and CDK2 were increased concomitant with the induction of DNA synthetic activity in outer medullae, but not in glomeruli, in adult kidneys during DNA synthetic period after ischemic injury. The p27(KIP1) protein, but not the p21(CIP1) protein, increased equally in total kidney, glomeruli, and outer medullae after ischemic injury. These results indicate that renal tubules have an active cyclin/CDK system, while glomeruli, do not have a cyclin/CDK system during active regeneration of kidneys after ischemic injury. Copyright (C) 2000 S. Karger AG, Basel.
Publisher
KARGER
Issue Date
2000-11
Language
ENG
Keywords

CYCLIN-DEPENDENT KINASES; CELL NUCLEAR ANTIGEN; INHIBITOR P27(KIP1); CANCER; REGENERATION; NEPHROGENESIS; EXPRESSION; REPAIR; MUSCLE

Citation

NEPHRON, v.86, pp.306 - 314

ISSN
0028-2766
DOI
10.1159/000045786
URI
http://hdl.handle.net/10203/73428
Appears in Collection
MSE-Journal Papers(저널논문)
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