Suppression of IL-2 and IL-4 gene expression by nodularin through the reduced NF-AT binding activity

Cited 13 time in webofscience Cited 0 time in scopus
  • Hit : 345
  • Download : 0
Nodularin is a cyclic peptide produced by cyanobacteria. In the present study, the inhibitory effect of nodularin on T lymphocyte functions was demonstrated. Direct addition of nodularin to B6C3F1 mouse splenocyte cultures produced a concentration-dependent inhibition of the lymphoproliferative response to concanavalin A stimulation. Nodularin inhibited PMA plus ionomycin (Io)-induced IL-2 mRNA expression in murine splenocytes and thymocytes as determined by quantitative/competitive RT-PCR. To further characterize the mechanism for the transcriptional regulation of IL-2, the binding activity of transcription factors, NF-AT, AP-1, NF-kappa B, and Oct, was evaluated by electrophoretic mobility shift assays in mouse splenocytes. Nodularin reduced the NF-AT binding activity in PMA/Io-induced splenocytes, but no significant effect was observed on AP-1, NF-kappa B, or Oct binding activity. Nodularin also inhibited IL-4 mRNA expression in PMA/Io-stimulated murine splenocytes. These results suggest that T lymphocyte is a possible cellular target of nodularin, and the inhibitory effect of nodularin on T-cell specific transcription factor NF-AT induces T-cell dysfunction, which leads to a diminution in IL-2 and IL-4 gene transcription. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
Publisher
Elsevier Ireland Ltd
Issue Date
2000
Language
English
Article Type
Article
Keywords

PROTEIN PHOSPHATASES; OKADAIC ACID; ADENYLATE-CYCLASE; POTENT INHIBITOR; CYCLOSPORINE-A; NUCLEAR FACTOR; GROWTH-FACTOR; MOUSE SKIN; PROMOTER; INTERLEUKIN-2

Citation

TOXICOLOGY LETTERS, v.114, no.1-3, pp.215 - 224

ISSN
0378-4274
URI
http://hdl.handle.net/10203/72893
Appears in Collection
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 13 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0