ADSORPTIVE REMOVAL OF INHIBITORY BY-PRODUCT IN THE ENZYMATIC PRODUCTION OF OPTICALLY-ACTIVE D-P-HYDROXYPHENYLGLYCINE FROM 5-SUBSTITUTED HYDANTOIN

One-step enzymatic production of D-p-hydroxyphenylglycine (D-HPG) from 5-substituted hydantoin was carried out using a bacterium Agrobacterium sp I-671 which possesses D-hydantoinase and N-carbamoylase. From the inhibition study, it was found that N-carbamoylase was severely inhibited by ammonium ions which is co-produced with D-HPG. In order to increase the conversion yield of D-HPG, simultanious removal of inhibitory by-product from reaction mixture was carried out using the specific adsorbents for ammonium ions. The conversion yield of D-HPG reached about 98% in the presence of adsorbents in 27 hours, while 50% conversion was observed in the absence of adsorbents.
Publisher
CHAPMAN HALL LTD
Issue Date
1994-01
Language
ENG
Keywords

D-AMINO ACIDS; MICROBIAL TRANSFORMATION

Citation

BIOTECHNOLOGY LETTERS, v.16, no.1, pp.17 - 22

ISSN
0141-5492
URI
http://hdl.handle.net/10203/67211
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
  • Hit : 146
  • Download : 0
  • Cited 0 times in thomson ci
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡClick to seewebofscience_button
⊙ Cited 9 items in WoSClick to see citing articles inrecords_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0