ADSORPTIVE REMOVAL OF INHIBITORY BY-PRODUCT IN THE ENZYMATIC PRODUCTION OF OPTICALLY-ACTIVE D-P-HYDROXYPHENYLGLYCINE FROM 5-SUBSTITUTED HYDANTOIN

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One-step enzymatic production of D-p-hydroxyphenylglycine (D-HPG) from 5-substituted hydantoin was carried out using a bacterium Agrobacterium sp I-671 which possesses D-hydantoinase and N-carbamoylase. From the inhibition study, it was found that N-carbamoylase was severely inhibited by ammonium ions which is co-produced with D-HPG. In order to increase the conversion yield of D-HPG, simultanious removal of inhibitory by-product from reaction mixture was carried out using the specific adsorbents for ammonium ions. The conversion yield of D-HPG reached about 98% in the presence of adsorbents in 27 hours, while 50% conversion was observed in the absence of adsorbents.
Publisher
CHAPMAN HALL LTD
Issue Date
1994-01
Language
ENG
Article Type
Article
Appears in Collection
BS-Journal Papers(저널논문)
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