cis-elements involved in alternative splicing in the rat beta-tropomyosin gene: the 3-splice site of the skeletal muscle exon 7 is the major site of blockage in nonmuscle cells

We have been using the rat beta-tropomyosin (beta-TM) gene as a model system to study the mechanism of alternative splicing. The beta-TM gene spans 10 kb with 11 exons and encodes two distinct isoforms, namely skeletal muscle beta-TM and fibroblast TM-1. Exons 1 - 5, 8, and 9 are common to all mRNAs expressed from this gene. Exons 6 and 11 are used in fibroblasts, as well as in smooth muscle cells, whereas exons 7 and 1 0 are used exclusively in skeletal muscle cells. Our previous studies localized the critical elements for regulated alternative splicing to sequences within exon 7 and the adjacent upstream intron. We also demonstrated that these sequences function, in part, to regulate splice-site selection in vivo by interacting with cellular factors that block the use of the skeletal muscle exon in nonmuscle cells (1). Here we have further characterized the critical cis-acting elements involved in alternative splice site selection. Our data demonstrate that exon 7 and its flanking intron sequences are sufficient to regulate the suppression of exon 7 in nonmuscle cells when flanked by heterologous exons derived from adenovirus. We have also shown by both in vivo and in vitro assays that the blockage of exon 7 in nonmuscle cells is primarily at its 3'-splice site. A model is presented for regulated alternative splicing in both skeletal muscle and nonmuscle cells.
Publisher
OXFORD UNIV PRESS
Issue Date
1993-10
Language
ENG
Keywords

PRE-MESSENGER-RNA; BRANCH POINT LOCATION; SEQUENCES; INVITRO; SELECTION; TRANSCRIPTS; INVIVO; POLYADENYLATION; IDENTIFICATION; BINDING

Citation

NUCLEIC ACIDS RESEARCH, v.21, no.20, pp.4762 - 4768

ISSN
0305-1048
DOI
10.1093/nar/21.20.4762
URI
http://hdl.handle.net/10203/65654
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
  • Hit : 109
  • Download : 0
  • Cited 0 times in thomson ci
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡClick to seewebofscience_button
⊙ Cited 20 items in WoSClick to see citing articles inrecords_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0