IMMOBILIZATION CAN IMPROVE THE STABILITY OF HYBRIDOMA ANTIBODY PRODUCTIVITY IN SERUM-FREE MEDIA

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Hybridoma cells (S3H5/γ2bA2) were cultivated in spinner flasks with 1% serum media and serum-free media. Monoclonal antibody productivity was maintained in 1% serum media. However, cells in serum-free media showed a decrease in antibody productivity, and it completely disappeared in IMDM-based low protein medium. This loss of antibody productivity was not observed when the cells were immobilized in alginate beads. In fact, immobilization enhanced the specific MAb productivity.
Publisher
Wiley-VCH Verlag
Issue Date
1990-12
Language
English
Article Type
Article
Citation

BIOTECHNOLOGY AND BIOENGINEERING, v.36, no.10, pp.1049 - 1055

ISSN
0006-3592
DOI
10.1002/bit.260361010
URI
http://hdl.handle.net/10203/64785
Appears in Collection
BS-Journal Papers(저널논문)
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