Microfluidic self-assembly of insulin monomers into amyloid fibrils on a solid surface

We report the self-assembly of insulin monomers into amyloid fibrils within microchannels. To demonstrate the microfluidic amyloid formation and fibril growth on a solid surface, we seeded the internal surfaces of the microchannels with insulin monomers via N-hydroxysuccinimide ester activation and continuously flushed a fresh insulin solution through the microchannels. According to our analysis using optical and fluorescence microscopy, insulin amyloid preferentially formed in the center of the microchannels and, after reaching a certain density, spread to the side walls of the microchannels. By using ex situ atomic force microscopy, we observed the growth of amyloid fibrils inside the microchannels, which occurred at a much higher rate than that in bulk systems. After 12 h of incubation, insulin formed amyloid spherulites having "Maltese cross" extinction patterns within the microchannels according to the polarized microscopic analysis. Microfluidic amyloid formation enabled low consumption of reagents, reduction of incubation time, and simultaneous observation of amyloid formation under different conditions. This work will contribute to the rapid analysis of amyloid formation associated with many protein misfolding diseases.
Publisher
AMER CHEMICAL SOC
Issue Date
2008-07
Language
ENG
Keywords

ALZHEIMERS-DISEASE; BOVINE INSULIN; MECHANISM; TEMPLATE; SPHERULITES; MICROSCOPY

Citation

LANGMUIR, v.24, no.14, pp.7068 - 7071

ISSN
0743-7463
DOI
10.1021/la800907c
URI
http://hdl.handle.net/10203/5808
Appears in Collection
BiS-Journal Papers(저널논문)MS-Journal Papers(저널논문)
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