사람 가스트린/SV40 Large T 항원의 융합 유전자가 도입된 형질전환 마우스 Transgenic Mice Carrying Human Gastrin/SV 40 Large T Antigen Fusion Genes

Tissue-specific tumorigenesis can be induced in transgenic mice by the directed expression of SV40 large T antigen. In an attempt to induce the tumors or cancers in stomach tissues, nine transgenic mice were genrated that harbored a chimeric gene composed of the SV40 large T antigen gene fused to the exon II of a human gastrin gene including 5 and 3 flanking sequences- It was represented through germ-line transmission that three transgene mice were mosaic. The transgene was expressed in all tissues examined by reverse transcription DNA polymerase chain reaction (RT-PCR) whereas endogenous mouse gastrin transcripts were detected only in both stomach and brain tissues of the transgenic mouse. The antral region of transgenic mice (TEX-3 line) exhibited abnormal state in appearance although stomach sections of transgenic mice have yet to show any histological or pathological changes.
Publisher
한국실험동물학회
Issue Date
1994
Language
KOR
Citation

한국실험동물학회지, v.10, no.2, pp.185 - 196

ISSN
1225-813x
URI
http://hdl.handle.net/10203/57595
Appears in Collection
MSE-Journal Papers(저널논문)
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