Mitochondrial dysfunction in Drosophila PINK1 mutants is complemented by parkin

Autosomal recessive juvenile parkinsonism (AR-JP) is an earlyonset form of Parkinson's disease characterized by motor disturbances and dopaminergic neurodegeneration(1,2). To address its underlyingmolecular pathogenesis, we generated and characterized loss-of-function mutants of Drosophila PTEN-induced putative kinase 1 (PINK1)(3), a novel AR-JP-linked gene(4). Here, we show that PINK1 mutants exhibit indirect flight muscle and dopaminergic neuronal degeneration accompanied by locomotive defects. Furthermore, transmission electron microscopy analysis and a rescue experiment with Drosophila Bcl-2 demonstrated that mitochondrial dysfunction accounts for the degenerative changes in all phenotypes of PINK1 mutants. Notably, we also found that PINK1 mutants share marked phenotypic similarities with parkin mutants. Transgenic expression of Parkin markedly ameliorated all PINK1 loss-of-function phenotypes, but not vice versa, suggesting that Parkin functions downstream of PINK1. Taken together, our genetic evidence clearly establishes that Parkin and PINK1 act in a common pathway in maintaining mitochondrial integrity and function in both muscles and dopaminergic neurons.
Publisher
Nature Publishing Group
Issue Date
2006-06
Language
ENG
Keywords

SIGNALING PATHWAY; DISEASE; APOPTOSIS; STRESS

Citation

NATURE, v.441, no.7097, pp.1157 - 1161

ISSN
0028-0836
DOI
10.1038/nature04788
URI
http://hdl.handle.net/10203/5619
Appears in Collection
BS-Journal Papers(저널논문)
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