Differential signaling has become a popular choice for high-speed digital interconnection schemes on printed circuit boards (PCBs), offering superior immunity to crosstalk and external noise. However, conventional differential lines on PCBs still have unsolved problems, such as crosstalk and radiated emission. When more than two differential pairs run in parallel, a line is coupled to the line adjacent to it because all the lines are parallel in a fixed order. Accordingly, the two lines that constitute a differential pair are subject to the differential-mode crosstalk that cannot be canceled out by virtue of the differential signaling. To overcome this, we propose a twisted differential line (TDL) structure on a high-speed multilayer PCB by using a concept similar to a twisted pair in a cable interconnection. It has been successfully demonstrated by measurement and simulation that the TDL is subject to much lower crosstalk and achieves a 13-dB suppression of radiated emission, even when supporting a 3-Gb/s data rate.