ER71 acts downstream of BMP, Notch, and Wnt signaling in blood and vessel progenitor specification

Cited 191 time in webofscience Cited 0 time in scopus
  • Hit : 592
  • Download : 6
FLK1-expressing (FLK1(+)) mesoderm generates blood and vessels. Here, we show that combined BMP, Notch, and Wnt signaling is necessary for efficient FLK1(+) mesoderm formation from embryonic stem cells (ESCs). Inhibition of BMP, Notch, and Wnt signaling pathways greatly decreased the generation of FLK1(+) mesoderm and expression of the Ets transcription factor Er71. Enforced expression of ER71 in ESCs resulted in a robust induction of FLK1(+) mesoderm; rescued the generation of FLK1(+) mesoderm when blocked by BMP, Notch, and Wnt inhibition; and enhanced hematopoietic and endothelial cell generation. Er71-deficient mice had greatly reduced FLK1 expression, died early in gestation, and displayed severe blood and vessel defects that are highly reminiscent of the Flk1 null mouse phenotype. Collectively, we provide compelling evidence that ER71 functions downstream of BMP, Notch, and Wnt signals and regulates FLK1(+) mesoderm, blood, and vessel development.
Publisher
CELL PRESS
Issue Date
2008-05
Language
English
Article Type
Article
Keywords

EMBRYONIC STEM-CELLS; HEMATOPOIETIC DEVELOPMENT; TRANSCRIPTION FACTORS; ETS FAMILY; MOUSE; EXPRESSION; PROTEIN; DIFFERENTIATION; HEMANGIOBLAST; ACTIVATION

Citation

CELL STEM CELL, v.2, no.5, pp.497 - 507

ISSN
1934-5909
DOI
10.1016/j.stem.2008.03.008
URI
http://hdl.handle.net/10203/5160
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 191 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0