Effects of quality and developmental stage on the survival of IVF-derived bovine blastocysts cultured in vitro after freezing and thawing

Factors affecting viability of IVF-derived bovine blastocysts after freezing and thawing were investigated. A total of 1,101 ova matured and fertilized in vitro were cultured under 2 different conditions, 1) in TCM-199 on granulosa cell monolayers at 5% CO2 in air and 2) in synthetic oviduct fluid (SOF) medium without somatic cell support at 5% CO2, 5% O-2, 90% N-2. All blastocysts that developed from the 2 different culture systems were individually classified into 4 grades of embryo quality and were then frozen by conventional slow freezing. Developmental rates of the IVF-derived ova to blastocysts and the survival rates of the frozen-thawed blastocysts were not different between the SOF medium (16 and 49%) and the co-culture system (13 and 61%, respectively). Survival of frozen-thawed blastocysts was affected by embryo quality in both the SOF and co-culture systems (P<0.001). Blastocysts produced in vitro were also individually classified into 3 developmental stages and were then cultured for 3 d in the co-culture system with granulosa cells after freezing and thawing. There was a difference in the survival rate of frozen-thawed embryos between blastocyst developmental stages (early vs mid, P<0.05; mid vs expanded, P<0.01; early vs expanded, P<0.001). The post-thawing survival rate of blastocysts frozen at Day 7 (62%) of culture was higher compared with that of Day 8 (45%), but there was no difference in survival rate between Day 7 and 8 of culture. The results indicate that the quality and developmental stage of blastocysts are important factors influencing their survival after freezing and thawing.
Publisher
BUTTERWORTH-HEINEMANN
Issue Date
1994-09
Language
ENG
Keywords

FERTILIZED INVITRO; FOLLICULAR OOCYTES; CATTLE EMBRYOS; CUMULUS CELLS; CO-CULTURE; IN-VITRO; FROZEN; VITRIFICATION; MATURATION; CALVES

Citation

THERIOGENOLOGY, v.42, no.4, pp.645 - 654

ISSN
0093-691X
DOI
10.1016/0093-691X(94)90381-R
URI
http://hdl.handle.net/10203/4856
Appears in Collection
BS-Journal Papers(저널논문)
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