Mechanical characteristic study of cancerous and normal cell = 암과 정상 세포의 기계적 특성에 대한 연구

Traditionally, biochemical and genetic approach has been widely used in basic cellular research. However, recent studies have shown that cells have very interesting mechanical characteristics. It is, therefore, meaningful to study mechanical and physical factors that are important in characterizing the cellular behavior. This thesis aims to study the mechanical characteristics of cancerous and normal liver cells. First we characterize cell’s elastic modulus by indentation and adhesion property by tension experiments. For the indentation experiment, a MEMS based cellular indenter is developed. The cellular indenter consists of a probe, flexible beams with embedded electrodes and a permanent magnet and was fabricated using MEMS (Micro Electro-Mechanical System) technique. The device is then calibrated using a glass fiber of known mechanical and geometrical properties. With the use of this device, we propose cellular indentation experiments and modelling using Hertz approximation. Also, the responses of cellular adhesion property of normal and cancerous liver cells are investigated upon tensional stimulation. By stretching the PDMS substrate with cells seeded onto, we apply tensional effects on cells. Adhesion proteins such as E-cadherin and vinculin are characterized and analyzed using biological methods. This work will help us characterize the mechanical properties of cancerous and normal cells, and establish a reasonable step stone for future cancer detection research.
Advisors
Shin, Jennifer Hyung-jongresearcher신현정researcher
Publisher
한국과학기술원
Issue Date
2008
Identifier
296117/325007  / 020063631
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 기계공학전공, 2008.2, [ vi, 61 p. ]

Keywords

Cellular Stiffness; Cellular Adhesion; Cellular Indentation; Cellular Indentation; Mechanical Property; 세포 강성; 세포 접착력; 세포 국부자극; 세포 인장; 기계적 물성치; Cellular Stiffness; Cellular Adhesion; Cellular Indentation; Cellular Indentation; Mechanical Property; 세포 강성; 세포 접착력; 세포 국부자극; 세포 인장; 기계적 물성치

URI
http://hdl.handle.net/10203/45637
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=296117&flag=t
Appears in Collection
ME-Theses_Master(석사논문)
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