Adhesion and growth of endothelial cell on amphiphilic PU/PS IPN surface : Effect of amphiphilic balance and immobilized collagen

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Since natural blood vessels are lined with an endothelial cell (EC) monolayer, it is proposed that synthetic biomaterial surfaces be covered or seeded with endothelial cells for ideal nonthrombogenicity under normal conditions. The effects of the surface energy of hydrophilic-hydrophobic IPN on EC adhesion and growth were investigated. The human umbilical vein endothelial cells (HUVECs) were cultured on polyurethane (PU)/polystyrene (PS) IPN prepared by changing only the amphiphilic balance and controlling the microphase separated surface structure. The collagens were immobilized on the IPN surfaces for enhanced adhesion of HUVECs, and the morphology of the collagens immobilized highly depended on the surface energy of the IPNs. The stranded rope structure of the collagen molecules in the solution state was maintained only on the surface of the IPN with intermediate hydrophilicity. The adhesion and the proliferation of ECs on the nontreated IPN surfaces increased by increasing the hydrophobicity of the IPNs, and they were optimized on the collagen-treated IPN surface having an intermediate hydrophilicity. Finally, platelet adhesion was significantly reduced on the EC-hybridized surface of the IPNs. (C) 2002 Wiley Periodicals, Inc.
Publisher
Wiley-Blackwell
Issue Date
2002-03
Language
English
Article Type
Article
Keywords

SUBSTRATUM-BOUND FIBRONECTIN; PROTEIN ADSORPTION; POLYMER SURFACES; EXTRACELLULAR-MATRIX; POLYSTYRENE SURFACE; BIOLOGICAL-ACTIVITY; COATED POLYSTYRENE; PLATELET-ADHESION; CONTACT ANGLES; BIOCOMPATIBILITY

Citation

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, v.62, no.4, pp.613 - 621

ISSN
0021-9304
URI
http://hdl.handle.net/10203/4508
Appears in Collection
CBE-Journal Papers(저널논문)
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