One-and two-dimensional NMR investigation of the chemically synthesized DNA d(GGGTACCC)2 = 화학 합성된 DNA d(GGGTACCC)2에 관한 1차원 및 2차원 핵자기 공명 연구

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One- and two-dimensional proton NMR studies were reported on the self-complementary d(GGGTACCC) duplex. The resonances of the exchangeable protons were assigned by one-dimensional NOE difference spectra and the melting behavior of these imino protons was observed with line broadening and chemical shift change. The resonance of nearly all of the nonexchangeable protons of octamer duplex in solution were assigned by proton two-dimensional nuclear Overhauser enhancement spectroscopy(NOESY) and J-correlated spectroscopy (COSY). We obtained complete assignment for the adenine H8, guanine H8, cytosine H6 and H5, thymine H6 and $CH_3$, and the deoxyribose H1``, H2``, H2", H3``, H4`` resonances. These assignment were required for the analysis of two dimensional NOE and J-coupled data in terms of the solution structure of oligonucleotides. A set of short ($<4.5$) proton-proton distances defined for complementary d (GGGTACCC) octamer duplex were estimated from NOESY data set recorded as a function of mixing time. The conformation and dynamics of the selfcomplementary DNA octamer d (GGGTACCC) duplex was studied by means of 1H NMR and distance constraints.
Advisors
Choi, Byoung-Seokresearcher최병석researcher
Description
한국과학기술원 : 화학과,
Publisher
한국과학기술원
Issue Date
1992
Identifier
59924/325007 / 000901598
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 화학과, 1992.2, [ v, 66 p. ]

URI
http://hdl.handle.net/10203/32181
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=59924&flag=dissertation
Appears in Collection
CH-Theses_Master(석사논문)
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