Study on the electron transfer of ferrocene-modified self-assembled monolayers and its application for biosensor platform = 페로센기를 가지고 있는 자기조립단분자막의 전자전달에 대한 연구 및 바이오센서에의 응용

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Electrochemical detection method was widely used as a biosensor. It was allowed to make a fast, direct, cheap and able-to-be automated biosensor. Existing electrochemical sensors required complicated steps to attach an enzyme and a redox-active molecule to solve the overpotential problem in electrical signal due to additional layers of SAMs and biomolecules. In this study, we made a simple platform using a ferrocenyl undecanethiol $(Fc(CH_2)_{11}SH)$ as an electron transfer mediator and mercapto dodecanoic acid (MDA) for immobilization of biomolecules. We had developed the platform as an enzyme-linked electrochemical sensor to detect biomolecular recognitions. Avidin and biotin have the strongest non-covalent bonding, so the interaction was widely used to attach the biomolecule in biosensor. In this paper biospecific interaction of avidin to biotinylated monolayers was successfully detected by the subsequent association with biotinylated alkaline phosphatase. It is first paper about biosensor using $Fc(CH_2)_{11}SH$ as an electron transfer mediator.
Advisors
Kwak, Ju-hyounresearcher곽주현researcher
Description
한국과학기술원 : 화학과,
Publisher
한국과학기술원
Issue Date
2008
Identifier
302031/325007  / 020064051
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 화학과, 2008. 8., [ vii, 44 p. ]

Keywords

SAMs; ferrocene; biosensor; avidin; biotin; 자기조립단분자막; 페로센; 바이오센서; 아비딘; 바이오틴; SAMs; ferrocene; biosensor; avidin; biotin; 자기조립단분자막; 페로센; 바이오센서; 아비딘; 바이오틴

URI
http://hdl.handle.net/10203/32096
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=302031&flag=dissertation
Appears in Collection
CH-Theses_Master(석사논문)
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