Studies on the characteristics of affinity chromatography for the separation of chymotrypsin = Chymotrypsin 효소의 분리를 위한 친화 크로마토 그래피의 특성에 관한 연구

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For the separation of enzyme, the characteristics of affinity chromatography was studied. The system employed Sepharose CL-4B as the gel matrix, 4-phenylbutylamine as a ligand and $\alpha$-chymotrypsin as a solute. Moment analysis was used to determine the retention time in a column, in which the longitudinal dispersion in the mobile phase, the radial dispersion inside the porous spherical gel beads and the sorption on the internal surface of the gel bead are simultaneously taking place. In the constant pattern assumption the averaged volumetric coefficient of mass transfer was obtained and the breakthrough curve could be calculated by the implicit scheme of finite difference method. To analyse the effect of pH on the retention time, an ionization model was incorporated into the chromatographic model. Dependence of adsorption equilibrium constant and the retention time on pH were investigated. From the experimental data the approximate pK values would be obtained. Also the dependence of retention time on the ligand concentration was investigated.
Chung, In-Jae정인재
Description
한국과학기술원 : 화학공학과,
Publisher
한국과학기술원
Issue Date
1984
Identifier
64078/325007 / 000821167
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 화학공학과, 1984.2, [ vii, 84 p. ]

URI
http://hdl.handle.net/10203/30174