In silico experiments and applications of recombinant escherichia coli = 재조합 대장균 균주 개발에서의 in silico 실험과 그 응용에 관한 연구

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In this study, a novel in silico experiments for the systems biotechnological strategy towards the development of improved strains is proposed. Previous wet experimental studies have indicated that the enhanced production of commodity chemicals in metabolically engineered E. coli can be achieved by a variety of strategies based on genetic alterations and environmental changes. In the current study, such strategies are applied for production of succinic acids and are verified by resorting to a constraints-based flux analysis which provides more information on the internal flux distribution of the engineered E. coli. This process is initiated by constructing a genome-scale in silico model of recombinant E. coli, and then followed by quantifying metabolic fluxes in response to the flux variations for each amplified or deleted gene. The resultant flux distributions render it possible to investigate the effect of genetic perturbation, i.e., gene deletion, attenuation and enhancement, on internal flux patterns of E. coli. Finally, target genes for commodity chemicals were identified by analyzing the internal flux patterns. Possibly, the production of succinic acid can be more enhanced by appropriately manipulating such essential fluxes identified. Thus, the in silico experiments can provide a new insight into the metabolic engineering strategy for achieving higher succinic acid productivity.
Advisors
Lee, Sang-Yupresearcher이상엽researcher
Description
한국과학기술원 : 생명화학공학과,
Publisher
한국과학기술원
Issue Date
2005
Identifier
249591/325007  / 020033165
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2005.8, [ ii, 44 p. ]

Keywords

metabolic flux analysis; Metabolic engineering; escherichia coli; 대장균; 대사흐름분석; 대사공학

URI
http://hdl.handle.net/10203/29880
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=249591&flag=dissertation
Appears in Collection
CBE-Theses_Master(석사논문)
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