Influence of L-amino acids on the cell growth and protein expression of eshcerichia coli W3110 = L-아미노산이 escherichia coli W3110의 세포성장과 단백질 발현에 미치는 영향

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The influences of L-amino acids on the cell growth and protein expression were investigated through the cultivation of E. coli W3110 in the media containing L-amino acids of various compositions and concentrations. In the media with 20 L-amino acids of 0.03g/L concentration before the exhaustion of 20 L-amino acids, the specific growth rate of cell, $0.906h^{-1}$, was higher than that in the minimal media without L-amino acids, $0.6h^{-1}$. But after the exhaustion of added L-amino acids, the specific growth rate, $0.492h^{-1}$, was lower than that in the minimal media, $0.6h^{-1}$. The optimal cell yield per unit glucose, 0.488 g/g, was obtained when 20 L-amino acids of 0.03g/L concentration were supplemented to the minimal media. This cell yield value was higher than that in the minimal without 20 L-amino acids, 0.445g/g. This demonstrates that considerable amounts of compounds serve as the carbon source. Also this value was higher than those in the abundant media containing 0.05g/L and 0.1g/L 20 L-amino acids, 0.450g/g and 0.402g/g, respectively. This result was obtained because the acetate was produced more in the media with more 20 L-amino acids. Among 20 L-amino acids, the only glycine could improve the specific growth rate of cell. When 20 L-amino acids mixture of 0.03g/L concentration were supplemented to the minimal media, R/2 media containing glucose, the specific growth rate between 3hour and 4hour suddenly decreased. The exhaustion of glycine had the most effect on this phenomenon. L-valine inhibited the cell growth by obstructing the synthesis of L-isoleucine. When L-valine above 0.00005 g/L was added individually, the time of lag phase was lengthened. But after a certain period, the cell growth (growth rate, $Y_{X/S}$, and $Y_{ace/X}$) was similar with that in the media without L-valine. In other words the cell growth could be restored by itself. The more L-valine was added, the longer the time of lag phase was. But at the concentration above 0...
Advisors
Chang, Ho-Namresearcher장호남researcher
Description
한국과학기술원 : 생명화학공학과,
Publisher
한국과학기술원
Issue Date
2005
Identifier
244222/325007  / 020033384
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2005.2, [ vi, 71 p. ]

Keywords

L-amino acids; Escherichia coli W3110; Escherichia coli W3110; L-아미노산

URI
http://hdl.handle.net/10203/29872
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=244222&flag=dissertation
Appears in Collection
CBE-Theses_Master(석사논문)
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