Optimization of metabolic pathway by metabolic flux analysis = 대사 흐름 분석을 통한 대사 회로의 최적화

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Metabolic engineering is a powerful approach for optimization of fermentation processes for more efficient production of desired products. Metabolic engineering can be categorized three parts: genetic modification, analysis and design. Analytical techniques such as expression analysis, protein analysis and metabolic pathway analysis enabled more rational design for strain improvement and biomolecules production. Metabolic flux analysis, which allows calculation of the intracellular metabolic fluxes based on the stoichiometry of intracellular reactions and mass balances around the intracellular metabolites, has become an essential tool for metabolic engineering. In this study, metabolic flux analyses were carried out to optimize metabolic pathways for maximum production of desired products. The optimized metabolic pathways for production of 6 metabolites (acetic acid, lactic acid, malic acid, pyruvic acid, succinic acid, ethanol) and 3 amino acids (aspartic acid, phenylalanine, glutamic acid) were predicted by metabolic flux analyses. Based on optimized metabolic pathways, new efficient metabolite production systems could be suggested, and enhanced production of metabolites might be achieved.
Advisors
Lee, Sang-Yupresearcher이상엽researcher
Description
한국과학기술원 : 생명화학공학과,
Publisher
한국과학기술원
Issue Date
2003
Identifier
180310/325007 / 020013210
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2003.2, [ iv, 61 p. ]

Keywords

Metabolic Flux Analysis; 대사 흐름 분석

URI
http://hdl.handle.net/10203/29774
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=180310&flag=dissertation
Appears in Collection
CBE-Theses_Master(석사논문)
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