Development of corynebacterium glutamicum whole cell bioconversion process for the production of cinnamaldehyde

Abstract

The necessity of developing a bio-based eco-friendly production system is emerging. Because the existing production methods of cinnamaldehyde, such as direct extraction or chemical synthesis, are not suitable for mass production and cause environmental problems. The production of cinnamaldehyde can be obtained by converting from L-phenylalanine to cinnamic acid through phenylalanine ammonia lyase and converting cinnamic acid to cinnamaldehyde using carboxylic acid reductase. Using strains expressing phenylalanine ammonia lyase and carboxylic acid reductase in Corynebacterium glutamicum as a whole cell catalyst, L-phenylalanine produced in the high-producing Escherichia coli strain is used as a precursor to produce cinnamaldehyde. The whole cell biocatalyst of each conversion step was reused using a tangential flow filtration module and alginate beads to increase the production efficiency of cinnamaldehyde. For L-phenylalanine, cinnamic acid, and cinnamaldehyde, productivity of 1.07 g/(L·h), 0.675 g/(L·h), and 0.47 g/(L·h) were obtained.