Non-destructive verification of PNA immobilization using click chemistry = Click chemistry를 이용한 PNA 고정화의 비파괴적 검사법

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 283
  • Download : 0
DC FieldValueLanguage
dc.contributor.advisorPark, Hyun-Gyu-
dc.contributor.advisor박현규-
dc.contributor.authorLim, Seong-Yoon-
dc.contributor.author임성윤-
dc.date.accessioned2011-12-13T01:47:51Z-
dc.date.available2011-12-13T01:47:51Z-
dc.date.issued2007-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=310371&flag=dissertation-
dc.identifier.urihttp://hdl.handle.net/10203/29474-
dc.description학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2007.2, [ vi, 36 p. ]-
dc.description.abstractThis study is designed for developing a fluorescent immobilization method using click chemistry allowing a direct and non-destructive verification of immobilization for PNA microarray. We used a chemical reaction synthesizing a fluorescent triazole compound through copper (I)-catalyzed 1,3-dipolar cycloaddition from non-fluorescent molecules. We firstly verified fluorescence of synthesized triazole molecule from combining azidocoumarin and phenyl-acetylene in solution state by UV-visible and photoluminescence spectroscopy. Spectroscopic results exhibited that the product has excitation and emission peak around at 360nm and 430 nm respectively. Identifying fluorescent feature of triazole compound, azidocoumarin-linked PNA was spotted on the phenyl acetylene modified glass and reacted in click condition. The fluorescence from immobilization and hybridization are separately confirmed by array scanner since the wavelength of the immobilized region is different from that of target-labeled fluorescence. This fluorescent immobilization can serve a cost-reducing quality control method for PNA microarray.eng
dc.languageeng-
dc.publisher한국과학기술원-
dc.subjectclick chemistry-
dc.subjectPNA-
dc.subjectimmobilization-
dc.subjectlfuorescent-
dc.subject클릭화학-
dc.subject펩타이드 핵산-
dc.subject고정화-
dc.subject형광-
dc.subjectclick chemistry-
dc.subjectPNA-
dc.subjectimmobilization-
dc.subjectlfuorescent-
dc.subject클릭화학-
dc.subject펩타이드 핵산-
dc.subject고정화-
dc.subject형광-
dc.titleNon-destructive verification of PNA immobilization using click chemistry = Click chemistry를 이용한 PNA 고정화의 비파괴적 검사법-
dc.typeThesis(Master)-
dc.identifier.CNRN310371/325007 -
dc.description.department한국과학기술원 : 생명화학공학과, -
dc.identifier.uid020053505-
dc.contributor.localauthorPark, Hyun-Gyu-
dc.contributor.localauthor박현규-
Appears in Collection
CBE-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0