Non-destructive verification of PNA immobilization using click chemistry = Click chemistry를 이용한 PNA 고정화의 비파괴적 검사법

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This study is designed for developing a fluorescent immobilization method using click chemistry allowing a direct and non-destructive verification of immobilization for PNA microarray. We used a chemical reaction synthesizing a fluorescent triazole compound through copper (I)-catalyzed 1,3-dipolar cycloaddition from non-fluorescent molecules. We firstly verified fluorescence of synthesized triazole molecule from combining azidocoumarin and phenyl-acetylene in solution state by UV-visible and photoluminescence spectroscopy. Spectroscopic results exhibited that the product has excitation and emission peak around at 360nm and 430 nm respectively. Identifying fluorescent feature of triazole compound, azidocoumarin-linked PNA was spotted on the phenyl acetylene modified glass and reacted in click condition. The fluorescence from immobilization and hybridization are separately confirmed by array scanner since the wavelength of the immobilized region is different from that of target-labeled fluorescence. This fluorescent immobilization can serve a cost-reducing quality control method for PNA microarray.
Advisors
Park, Hyun-Gyuresearcher박현규researcher
Description
한국과학기술원 : 생명화학공학과,
Publisher
한국과학기술원
Issue Date
2007
Identifier
310371/325007  / 020053505
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2007.2, [ vi, 36 p. ]

Keywords

click chemistry; PNA; immobilization; lfuorescent; 클릭화학; 펩타이드 핵산; 고정화; 형광; click chemistry; PNA; immobilization; lfuorescent; 클릭화학; 펩타이드 핵산; 고정화; 형광

URI
http://hdl.handle.net/10203/29474
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=310371&flag=dissertation
Appears in Collection
CBE-Theses_Master(석사논문)
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