In silico-based metabolic engineering of mannheimia succiniciproducens for succinic acid production = 맨하이미아 균의 대사회로 분석 및 대사공학에 의한 숙신산 생산 균주 개발

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Importance of C4 compounds has been mentioned for a long time because of their applications and usefulness in industry. There have been a lot of efforts to substitute microbial process of producing four carbon materials for current petroleum-based process expensive and harmful to environment. We describe a method for developing the homo-production of a valuable C4 compound, succinic acid, by metabolic engineering of Mannheimia succiniciproducens MBEL55E based on its in silico experimental data. Construction of in silico metabolic network model of M. succiniciproducens with the complete genome sequence database facilitated to target the genes to be manipulated, and to develop optimal chemical defined medium. In combination with this defined medium, the targeted strain M. succiniciproducens PALK $(ldhA::Km^{r} pta-ackA::Sp^{r})$ shows homo-production of succinic acid with trivial amount of by-products through the entire fermentation. This study provides a novel method of in silico-based metabolic engineering of microorganisms to achieve homo-production of a metabolite, and pulls up the competitiveness of microbial process in industrial biotechnology.
Advisors
Lee, Sang-Yupresearcher이상엽researcher
Description
한국과학기술원 : 생명화학공학과,
Publisher
한국과학기술원
Issue Date
2007
Identifier
310370/325007  / 020053504
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2007.2 , [ iii, 63 p. ]

Keywords

Mannheimia succiniciproducens; succinic acid; metabolic engineering; in silico metabolic network model; 맨하이미아 숙시니시프로듀센스; 숙신산; 대사 공학; 대사회로 분석; Mannheimia succiniciproducens; succinic acid; metabolic engineering; in silico metabolic network model; 맨하이미아 숙시니시프로듀센스; 숙신산; 대사 공학; 대사회로 분석

URI
http://hdl.handle.net/10203/29473
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=310370&flag=dissertation
Appears in Collection
CBE-Theses_Master(석사논문)
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