Development of nanobiosensors for biotechnological and environmental applications생물공학적 및 환경모니터링을 위한 나노바이오센서의 개발 연구

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(Nano) Biosensors are hybrid devices that transform chemical information into an analytically useful signal by means of a biochemical mechanism. Biosensors consist of a receptor system, in which a biological component interacts specifically with a given analyte, and a coupled physicochemical transducer that amplifies the signal resulting from such interaction from molecular up to macroscopical level. The purpose of these constructs is the identification, quantification and eventual screening of specific molecules, as present in complex mixtures from moderate to very low concentrations. Therefore, biosensors have utility in analytical research but also in clinical diagnosis, food and pharmaceutical industry, environmental control and process monitoring. The interest in biosensor development has partly arisen from the need of fast and routine analysis of a large number of samples, what requires robustness, sensitivity and reproducibility. Essentially, these instruments can generate either optical or electric signals which are very convenient for instrument development, and the commercial application of many biosensors in research. Work on biosensors has focused mostly on enzyme based sensors, receptor based sensor, nucleic acid based sensors, immunosensors, and microbial sensors. In Chapter 2, we examined the possibility of in situ enzymatic reaction around the SWNTs. As a model reaction system, we selected the enzyme, cyclodextrin glucanotransferase (CGTase), which allows synthesis of cyclodextrins (CDs) from starch. CDs are able to form inclusion complexes with hydrophobic insoluble molecules via host-guest recognition and thereby solubilizing them in aqueous solution. Therefore, we decided to examine whether in situ CD formation around SWNTs from starch by CGTase will result in solubilization of SWNTs. Here, we report successful in situ CGTase reaction on the surface of SWNTs in aqueous solution, and subsequent formation of interesting macrocyclic supramolecu...
Advisors
Lee, Sang-Yupresearcher이상엽researcher
Description
한국과학기술원 : 생명화학공학과,
Publisher
한국과학기술원
Issue Date
2004
Identifier
240690/325007  / 020015835
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명화학공학과, 2004.8, [ xiv, 149 p. ]

Keywords

탄수화물칩; 자기조립; 탄소나노튜브; 나노바이오센서; 마이크로컨텍트 프린팅; MICROCONTACT PRINTINGATION; CARBOHYDRATE CHIP; QUANTUM DOTS; SELF-ASSEMBLY; CARBON NANOTUBES; NANOBIOSENSORS

URI
http://hdl.handle.net/10203/28937
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=240690&flag=dissertation
Appears in Collection
CBE-Theses_Ph.D.(박사논문)
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