DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Chung, Jae-Hoon | - |
dc.contributor.advisor | 정재훈 | - |
dc.contributor.author | Park, Jae-Ho | - |
dc.contributor.author | 박재호 | - |
dc.date.accessioned | 2011-12-12T09:01:46Z | - |
dc.date.available | 2011-12-12T09:01:46Z | - |
dc.date.issued | 1998 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=135362&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28555 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물과학과, 1998.2, [ v, 35 p. ] | - |
dc.description.abstract | Mouse GABA A receptor beta 3 subunit gene (Gabrb3) is located at the imprinted region on chromosome 7. To detect a possible biased parental expression from Gabrb3 gene, a dimorphic PstI restriction enzyme site was used for restriction fragment length polymorphism (RFLP) analysis. RFLP analysis of PCR-amplified Gabrb3 cDNA from brain tissue of hybrid mice proved that Gabrb3 gene is not imprinted. Quantitative analysis of allelic expression of Gabrb3 showed that DBA/2 allele was more expressed than C57BL/6 allele in (C57BL/6 × DBA/2)F1 and (DBA/2 × C57BL/6)F1 hybrid mice indicating that Gabrb3 gene expression is influenced by strain-of-origin rather than by parent-of-origin For discrimination and quantitation of transcripts from homologous alleles without RFLP, allele specific termination (AST) analysis was developed. Control experiments with GABA A receptor gamma 2 subunit gene (Gabrg2) proved that AST analysis is highly sensitive for the minor allele transcript representing over 10% of total transcripts of a gene. Application of AST analysis revealed that Fas gene is equally expressed from both parental alleles in (C57BL/6 × BALB/C)F1 hybrid mouse. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Mouse | - |
dc.subject | Epigenetic | - |
dc.subject | GABA A Receptor Beta 3 | - |
dc.subject | Gene expression | - |
dc.subject | GABA A Receptor Beta 3 유전자 | - |
dc.subject | 유전자 발현 | - |
dc.subject | 생쥐 | - |
dc.subject | 후유전적 | - |
dc.title | Epigenetic control of GABA A receptor Beta3 gene expression in mouse brain | - |
dc.title.alternative | GABA A Receptor Beta 3 유전자의 생쥐 뇌에서 후유전적 발현 조절 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 135362/325007 | - |
dc.description.department | 한국과학기술원 : 생물과학과, | - |
dc.identifier.uid | 000963257 | - |
dc.contributor.localauthor | Chung, Jae-Hoon | - |
dc.contributor.localauthor | 정재훈 | - |
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