Epigenetic control of GABA A receptor Beta3 gene expression in mouse brain = GABA A Receptor Beta 3 유전자의 생쥐 뇌에서 후유전적 발현 조절

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Mouse GABA A receptor beta 3 subunit gene (Gabrb3) is located at the imprinted region on chromosome 7. To detect a possible biased parental expression from Gabrb3 gene, a dimorphic PstI restriction enzyme site was used for restriction fragment length polymorphism (RFLP) analysis. RFLP analysis of PCR-amplified Gabrb3 cDNA from brain tissue of hybrid mice proved that Gabrb3 gene is not imprinted. Quantitative analysis of allelic expression of Gabrb3 showed that DBA/2 allele was more expressed than C57BL/6 allele in (C57BL/6 × DBA/2)F1 and (DBA/2 × C57BL/6)F1 hybrid mice indicating that Gabrb3 gene expression is influenced by strain-of-origin rather than by parent-of-origin For discrimination and quantitation of transcripts from homologous alleles without RFLP, allele specific termination (AST) analysis was developed. Control experiments with GABA A receptor gamma 2 subunit gene (Gabrg2) proved that AST analysis is highly sensitive for the minor allele transcript representing over 10% of total transcripts of a gene. Application of AST analysis revealed that Fas gene is equally expressed from both parental alleles in (C57BL/6 × BALB/C)F1 hybrid mouse.
Advisors
Chung, Jae-Hoonresearcher정재훈researcher
Description
한국과학기술원 : 생물과학과,
Publisher
한국과학기술원
Issue Date
1998
Identifier
135362/325007 / 000963257
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물과학과, 1998.2, [ v, 35 p. ]

Keywords

Mouse; Epigenetic; GABA A Receptor Beta 3; Gene expression; GABA A Receptor Beta 3 유전자; 유전자 발현; 생쥐; 후유전적

URI
http://hdl.handle.net/10203/28555
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=135362&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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