Isolation of chemotactic signalling mutants in escherichia coli = Escherichia coli에서 주화성과 관련된 신호발생 돌연변이체의 분리

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Bacterial chemotaxis is the response of an organism to a chemical gradient. Chemotactic sensory transducers located in the cytoplasmic membrane sense the stimulus and change the flagellar rotation frequency through the cytoplasmic sensory transduction pathway. Trg is the transducer for ribose and galactorse. For the purpose of understanding the signalling domain of Trg, the trg gene on a multicopy plasmid was mutagenized by hydroxylamine in vitro. After screening on chemotactic swarm plate, three mutant plasmids which show generally nonchemotactic phenotypes were isolated. They did``nt show any chemotactic activity on multicopy plasmid but gave slight response to ribose and galactose in a single chromosomal copy like previously characterized signalling mutation trg-21. The proteins from the mutations, named trg-202 and trg-203, show abnormal mobility on SDS-PAGE resulting from the defect in the deamidation like trg-21 In contrast, another mutation trg-201 is able to undergo deamidation in the same manner as wild type Trg protein. The mutations, trg-202 and trg-203, exhibit a pronounced counterclockwise bias in flagellar rotation while trg-201 shows exclusively clockwise rotation.
Advisors
Park, Chan-Kyuresearcher박찬규researcher
Description
한국과학기술원 : 생물공학과,
Publisher
한국과학기술원
Issue Date
1988
Identifier
66129/325007 / 000861272
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1988.2, [ iii, 37 p. ]

Keywords

세포 신호 전달.

URI
http://hdl.handle.net/10203/28287
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=66129&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
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