Production and characterization of monoclonal antibodies to portunus trituberculatus hemocyanin = Portunus trituberculatus hemocyanin 에 대한 단일 클론 항체의 생성과 그 특성

Murine monoclonal antibodies to hemocyanin of the crab, Portunus trituberculatus was derived from somatic cell fusion between Balb/c immune splenocytes and myeloma. Six hybridoma lines were selected and cloned, each of them sectrets IgG 1 and exhibits titres around those of 500-1,000 fold diluted antisera. One of six monoclonal antibodies were extensively characterized and used for studying cognate antigen. Immunochemical relationship with arthropodan hemocyanin and effect on pseudo-peroxidatic activity were studied with conventional antiserum and monoclonal antibody. Six monoclonal antibodies were shown to be inhibitory in peroxidatic reaction of Portunus hemocyanin, anti-Portunus hemocyanin monoclonal antibody (POTE53) and serum was cross-reactive with keyhole limpet hemoyanin in competititive radioimmune assay whereas antisera of Portunus hemocyanin and keyhole limpet hemocyanin were not cross-reactive in double immunodiffusion.
Advisors
Yang, Kyu-Hwanresearcher양규환researcher
Publisher
한국과학기술원
Issue Date
1987
Identifier
65588/325007 / 000851268
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1987.2, [ vii, 64 p. ]

URI
http://hdl.handle.net/10203/28263
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=65588&flag=t
Appears in Collection
BS-Theses_Master(석사논문)
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