Chemical synthesis and cloning of gene for A and B chains of human insulin = 사람 인슐린 A 와 B 사슬 유전자의 화학합성과 클로닝

Total synthesis of human insulin genes, 104 and 80 base pairs long for the insulin A and B chains were designed for the expression in the bacterial systems, respectively. The synthetic DNA fragments were synthesized through the phosphite method on solid support. The enzymatic joinings of the oligonucleotides were employed to give the synthetic DNA duplexes, followed by gene cloning to the N-terminus of the beta-galactosidase gene in bacteriophage M13 mp19 and plasmid pUC19. In order to simplify the characterization of clones and the indentification procedures, a couple of DNA fragments encoding short peptides were inserted at the N-terminus of the genes. The usefulness of such devices in conjunction with the expression level of the insulin genes was discussed. Cloned synthetic DNA fragments were sequenced and known that the sequence and leading frame were correct.
Advisors
Byun, Si-MyungresearcherLee, Dae-Silresearcher변시명researcher이대실researcher
Publisher
한국과학기술원
Issue Date
1987
Identifier
65584/325007 / 000851133
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1987.2, [ x, 68 p. ]

URI
http://hdl.handle.net/10203/28259
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=65584&flag=t
Appears in Collection
BS-Theses_Master(석사논문)
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