Chemical approach for optimization of translation of β-galactosidase = 화학적 합성 DNA fragment 에 의한 translation 의 최적화

The two synthetic DNA duplexes, the one as control having an optimized sequence and the other possessing partially random sequence, corresponding to the region of ribosome binding site were synthesized through the phosphite method on solid support. The synthetic RBS DNA duplexes having Eco RI and HindIII cohesive ends were cloned into the previously engineered gap in plasmid pMKT2 possessing 1pp promoter and the $\beta$-galactosidase gene. This construction gave a direct screening system for the expression level on the plate by the difference of color intensities of colonies formed. The colonies which showed the; high expression of $\beta$-galactosidase are characterized in terms of the activities and sequence. The effects of the sequence on RBS region to the translational efficiency are discussed.
Advisors
Yoo, Ook-JoonresearcherLee, Dae-Silresearcher유욱준researcher이대실researcher
Publisher
한국과학기술원
Issue Date
1987
Identifier
65579/325007 / 000851124
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1987.2, [ vi, 50 p. ]

URI
http://hdl.handle.net/10203/28254
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=65579&flag=t
Appears in Collection
BS-Theses_Master(석사논문)
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