Studies on the two new restriction endonuclease, Cth Ⅰ and Cth Ⅱ, from clostridium thermocellum = 새로운 제한효소 Cth Ⅰ 과 Cth Ⅱ 에 관한 연구

A set of new restriction endonuclease, Cth I, Cth II, is isolated from Clostridium thermocellum ATCC27405 by the following steps: ammonium sulfate fractionation, DEAE-cellulose, phosphocellulose (P-11) column chromatography. Restriction endonuclease, CthI cleaves neither pUC9 nor pBR322 plasmid DNA, while it cleaves SV40 and T7 DNA at one site, Adeno2 DNA at five sites, bacteriophage lamda DNA at eight sites. Restriction endonuclease, CthII, cleaves M13 DNA at two sites, bacteriophage lamda DNA at seventy one sites, Adeno2 DNA at one hundred thirty six sites. The restriction enzyme, R-CthI shows maximum activity at pH 9 to 11, 0 mM Nacl, 2 to 20 mM $Mg^{++}$ and $65\,^\circ\!C$. Restriction enzyme, R-CthII shows maximum activity at pH 8 to 9, 0 mM NaCl, 10 to 20 mM $Mg^{++}$ and $55\,^\circ\!C$. Restriction endonuclease, Cth II recognize the same sequence as Bcl I 5``-TGATCA-3`` and the restriction endonuclease, Cth II recognize the same sequence as BstN I, EcoR II, Zan I 5``-CC$^A_T$GG-3``.
Advisors
Yoo, Ook-Joonresearcher유욱준researcher
Publisher
한국과학기술원
Issue Date
1986
Identifier
65054/325007 / 000841334
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1986.2, [ 60 p. ]

Keywords

단백질 분리.

URI
http://hdl.handle.net/10203/28245
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=65054&flag=t
Appears in Collection
BS-Theses_Master(석사논문)
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