Construction of dimeric-vector system for efficient cloning of cDNA = 효율적인 cDNA 클로닝을 위한 dimeric-vector 계의 제조

Dimeric vector was constructed from pUC 18 and pUC 19 for more simplified and efficient cDNA cloning. This system has characteristics which are: 1) vector itself serves as primer for cDNA synthesis, 2) Sl nuclease treatment is not required, 3) vector-primer and linkers are rapidly and reliably prepared. With RNA size fractionation, this system will accomplish an efficient and simplified cDNA cloning system of bovine liver mRNA. Bovine liver mRNA was prepared from fresh liver tissues and proved to have good proved to have good translational activity. Prothrombin-specific mRNA was identified in the bovine liver mRNA preparation by northern blot hybridization with $^{32}$p-oligo nucleotide containing a segment of complementary sequences of prothrombin mRNA sequences.
Advisors
Han, Moon-Hi한문희
Publisher
한국과학기술원
Issue Date
1986
Identifier
65036/325007 / 000841068
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1986.2, [ [iv], 54 p. ]

URI
http://hdl.handle.net/10203/28227
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=65036&flag=t
Appears in Collection
BS-Theses_Master(석사논문)
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