DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Yoo, Ook-Joon | - |
dc.contributor.advisor | 유욱준 | - |
dc.contributor.author | Whang, Young | - |
dc.contributor.author | 황영 | - |
dc.date.accessioned | 2011-12-12T08:56:50Z | - |
dc.date.available | 2011-12-12T08:56:50Z | - |
dc.date.issued | 1985 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=64513&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/28222 | - |
dc.description | 학위논문(석사) - 한국과학기술원 : 생물공학과, 1985.2, [ 43 p. ] | - |
dc.description.abstract | TypeII restriction and modification enzymes are ideal model systems for studying the specific DNA-protein interaction because of their small size, simple catalytic requirements for activity and recognition sequence specificity. A new restriction endonuclease, XciI, has been partially purified from Xanthomonas citri IFO 3835, it catalytic properties and recognition sequence have been identified. This enzyme XciI cleaves bacteriophage $\lambda$DNA at two sites, pUC9 and pBR322 plasmid DNA at one site, but it cleaves neither $\phi$X174 DNA nor SV40 DNA. The enzyme shows maximum activity at pH values between 7.5 and 9.0, and in the presence of 20 mM MgCl$_2$. The addition of large amounts of Sodium Chloride ($>100mM$) causes a decrease in XciI activity. Temperature for optimum endonuclease activity is $37\,^\circ\!C$. But, Bovine Serum Albumin and sulfhydryl compounds have no influences on XciI restriction endonuclease activity. This restriction endonuclease XciI recognizes the sequence 5``-GTCGAC-3`` 3``-CAGCTG-5`` and cuts at the sites indicated by the arrows, identical with that cleaved by endonuclease SaII (of Streptomyces albus G). | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 단백질 분리. | - |
dc.title | (The) purification and characterization of a new type Ⅱ restriction endonuclease, XciⅠ | - |
dc.title.alternative | XciⅠ 제한 효소의 정제 및 분자적 특성에 관한 연구 | - |
dc.type | Thesis(Master) | - |
dc.identifier.CNRN | 64513/325007 | - |
dc.description.department | 한국과학기술원 : 생물공학과, | - |
dc.identifier.uid | 000831444 | - |
dc.contributor.localauthor | Yoo, Ook-Joon | - |
dc.contributor.localauthor | 유욱준 | - |
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