(The) optimal condition and possible regulatory factor for IL-2 production in mouse thymoma EL-4 cell = 쥐의 흉선종양 세포인 EL-4 세포에서 IL-2(interleukin-2) 생성최적화 및 조절요인에 대한 연구

The conditions for the production of a T cell growth factor (TCGF or IL-2) capable of maintaining the long-term growth of activated lymphocytes and present in the supernatants of mouse stimulated EL-4 thymoma cells were investigated. IL-2 was induced in spleen cells and various T cell lines stimulated with T cell lectins and/or PMA (phorbol-12-myristate-13-acetate). The kinetics of IL-2 production by EL-4 cells were highly dependent on the cellular concentrations, concentration of serum, and inducers in the culture media, and especially incubation time. The maximum activity of IL-2 appeared in the early phase with high cell concentration and in the late phase with low concentration of cells. The kinetic study of IL-2 production in EL-4 cells demonstrated the maximum activity of IL-2 at 24hr incubation and the subsequent dramatic decrease was observed in the late phase of the culture. The rate of IL-2 production also appeared in the same pattern. The arrest of IL-2 activity in the late phase seemed to be due to plausible types of regulatory mechanism ; the depletion of some essential components in the induction system, feedback inhibition by IL-2 formed in the early phase of the culture, and IL-2 absorption by EL-4 cells in the different phase of the culture. The possible regulatory factors will be discused.
Advisors
Han, Moon-Hi한문희
Publisher
한국과학기술원
Issue Date
1985
Identifier
64509/325007 / 000831657
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1985.2, [ v, 59 p. ]

URI
http://hdl.handle.net/10203/28218
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=64509&flag=t
Appears in Collection
BS-Theses_Master(석사논문)
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