Hydrolysis of triacylglyceride by the lipase from candida rugosa in isooctane-aot reversed micelle = 이소옥테인-에이.오.티 역 미셀계에서 Candida rugosa 로 부터 얻은 리파-제에 의한 트리글리세라이드의 가수분해

The hydrolysis of triacylglyceride by reversed micelle of the lipase was studied. The enzyme was solubilized in reversed micelle of bis (2-ethylhexyl) sodium sulfosuccinate (25 mM, AOT) in isooctane and water (0.31\%). The enzyme activity depended on the molar ratio of water to AOT (R value), and maximum activity was observed when the ``R value`` was 7. It is remarkable that under the conditions investigated, the reaction rate in the micelle was ca. 6 times faster than that in aqueous emulsion system. The pH-activity profile showed that optimum pH was 7.3 in isooctane-AOT micellar solution. Optimum temperature was at $30-35\,^\circ\!C$. Also, Ea, activation energy was 7.9 Kcal/mole. When ``R`` value was seven, apparent Km and Vmax for olive oil were 0.80\% and 51 uM/mg/hr, respectively. In the enzyme stability against isooctane, the relative activity of the lipase entrapped in AOT-isooctane rapidly dropped to ca. 50\% after 2 hours. The order of the reaction complied with 2nd order reaction.
Advisors
Rhee, Joon-Shickresearcher이준식researcher
Publisher
한국과학기술원
Issue Date
1985
Identifier
64492/325007 / 000831109
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1985.2, [ iii, 43 p. ]

URI
http://hdl.handle.net/10203/28202
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=64492&flag=t
Appears in Collection
BS-Theses_Master(석사논문)
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