(The) use of primary hepatocytes culture to detect DNA single-strand breaks and unscheduled DNA synthesis induced by procarcinogens = 일차 배양 간세포를 이용한 발암물질에 의한 DNA 단사 절단과 비 주기성 DNA 합성의 측정

Procarcinogen induced DNA single-strand breaks and unscheduled DNA synthesis were measured in primary rat hepatocytes culture. For single-strand breaks assay, rat liver DNA was prelabeled by injecting $^3$H-thymidine during the peak of DNA synthesis following partial hepatectomy. Hepatocytes were isolated from the rat 2 week after surgery by collagenase perfusion technique and maintained in the hormonal medium AB. DNA single-strand breaks were measured by the alkaline elution technique. Aflatoxin B , 2-acetylaminofluorene and diethylnitrosamine made doseand time-related increases in the number of single-strand breaks. Unscheduled DNA synthesis was measured on the basis of incorporation of $^3$H-thymidine into DNA for carcinoge treated hepatocytes. DNA was purified by treating the cells with SDS-EDTA-glycine solution and proteinase K on 2 $\mu{m}$ pore size PVC filter. Aflatoxin B , 2-acetylaminofluorene and diethylnitrosamine also showed a dose-dependent unscheduled DNA synthesis response. These results showed a good correlation between two methods and alkaline elution was slightly more sensitive than was unscheduled DNA synthesis.
Advisors
Yang, Kyu-Hwanresearcher양규환researcher
Publisher
한국과학기술원
Issue Date
1984
Identifier
64037/325007 / 000821042
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생물공학과, 1984.2, [ [iv], 46 p. ]

URI
http://hdl.handle.net/10203/28157
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=64037&flag=t
Appears in Collection
BS-Theses_Master(석사논문)
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