High-throughput expression profiling and surface protein identification of bone-marrow derived mesenchymal stem cells = 고처리량 방법을 이용한 골수 간엽 줄기세포의 발현 프로파일링과 표면단백질의 탐색

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 231
  • Download : 0
Bone marrow-derived mesenchymal stem cells (BM-MSC) are promising candidates for cell therapy and tissue engineering because they can be expanded in-vitro and induced to differentiate into many cell types. However, MSC’s are yet poorly described by genome-wide analyses. A better understanding of their regulatory physiology is required for manipulating them in vitro. Especially, we yet lack specific surface proteins that can be used to select MSCs for isolation from tissues. Therefore, a genome-wide transcription profile of BM-MSC was obtained using cDNA microarrays, with peripheral-blood mononuclear cells as a baseline. In addition, we combined a molecular tagging approach with the widely used gel-based MALDI-TOF, and also did a series of liquid chromatography-tandem mass spectrometry analyses (LC-MS/MS) on membrane fractions obtained by differential centrifugation to analyze the surface subproteome of MSC’s, in comparison to MSC-derived adipocytes. We found that genes that were highly expressed in BM-MSC’s, which we termed the MSC-related molecular profile, was similar to MSC’s from umbilical cord blood (UCB-MSC), but different from both hematopoietic stem cells (HSC) and embryonic stem cells (ESC). The membrane subproteome of MSC’s also revealed many candidate surface markers.
Advisors
Koh, Gou-Youngresearcher고규영researcher
Description
한국과학기술원 : 생명과학과,
Publisher
한국과학기술원
Issue Date
2007
Identifier
264263/325007  / 020053019
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명과학과, 2007.2, [ viii, 49 p. ]

Keywords

proteomics; tandem mass spectrometry; mesenchymal stem cell; genomics; 유전체; 단백질체; 간엽줄기세포; microarray

URI
http://hdl.handle.net/10203/28085
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=264263&flag=dissertation
Appears in Collection
BS-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0